Parameters and Presets
Parameters
Recipe parameters for Phase Cell Tracking and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
|---|---|---|---|---|
Detection | Background Flattening Factor | 0 | 100 | Adjusts the sensitivity of the background flattening operation; a lower value will preserve larger objects and more background variations |
Contrast Threshold | 0 | 255 | Adjusts the detection sensitivity on the machine learning-enhanced image; a lower value will detect bigger and more objects | |
Fill Holes Size | 0 | 50,000 | Adjusts the maximum size threshold for filling in gaps inside a detected object; a lower value will preserve more holes in the detection | |
Smoothing Factor | 0 | 100 | Adjusts the amount of smoothing applied to the outline of the detected objects; a lower value will preserve more of the object's morphological features | |
Tracking | Object Size | 0 | 1,000,000 | Specifies the range of objects to be included in the analysis results based on the area of the detected objects |
Max Search Range | 0 | 1,000 | Specifies the maximum distance for track-point matchmaking between successive time frames; a higher value will expand the search distance for fast-moving cells | |
Min Track Length | 0 | 1,000 | Specifies the minimum number of time frames before a detected object is considered a valid track; a lower value will generate more, and often shorter, tracks | |
Separation Factor | 1 | 100 | Adjusts the sensitivity of the object separation operation, a lower value will preserve larger objects with multiple intensity peaks |
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