Parameters and Presets
Parameters
Recipe parameters for Particle Tracking and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
---|---|---|---|---|
Detection | Background Removal Factor | 0 | 100 | Adjusts the sensitivity of the background removal operation; a lower value will preserve larger objects and more background variations |
Object Enhancement Factor | 0 | 50 | Adjusts the sensitivity of the bright peak enhancement operation; a lower value will enhance smaller, individual peaks | |
Contrast Threshold | 0 | 255 | Adjusts the detection sensitivity on the background-removed image; a lower value will detect more objects | Tracking|
Motion vs Intensity | Motion vs Intensity | 0 | 10 | Adjusts the relative weighting between motion and object intensity for track-point matchmaking between successive frames; a value of 5 will apply equal weights to motion and intensity for matchmaking |
Tracking | Gap Length | 0 | 10 | Specifies the maximum number of time frames for linking tracks that are spatially colocated; a higher value will preserve particle tracks for longer |
Min Track Length | 0 | 50,000 | Specifies the minimum number of time frames before a detected object is considered a valid track; a lower value will generate more, and often shorter, tracks | |
Max Search Range | 0 | 50,000 ( px or µm) | Specifies the maximum distance for track-point matchmaking between successive time frames; a higher value will expand the search distance for fast-moving cells |
Presets
There are three preset groups in the recipe: Detection, Motion vs Intensity, and Tracking. Each group has three pre-configured parameter groupings to help you get started on the analysis. The default preset values are in the sections subsections to follow.
Tutorial (video)
Measurements
The Particle Tracking recipe generates intensity and track position measurements for each detected particle. You can add additional measurements to the analysis results by using with the Measurement Tool in Aivia and explore measurement definitions on the Measurement Definitions page. The measurements generated by the recipe are in the table below.
Intensity | Position |
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Image
creditsCredits
Laura Anne Lowery, Boston College, Boston MA; David Van Vactor, Harvard Medical School, Boston MA
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