Recipe parameters for Colony Analysis and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
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Colony Detection | Colony Detection Sensitivity | 0 | 255 | Adjusts the sensitivity of the detection; a lower value will detect bigger and more objects; the MEF substrate and Matrigel substrate options let you select different detection routines depending on your plating substrate |
Colony Refinement | Minimum Colony Area | 0 | 1,000,000,000 px2 or µm2 | Specifies the minimum size for objects to be included in the analysis results based on the areas of the detected objects |
Fill Holes Area | 0 | 10,000,000 px2 or µm2 | Adjusts the maximum size threshold for filling in gaps inside detected objects; a lower value will preserve more holes in the detection | |
Smoothing Factor | 0 | 100 | Adjusts the amount of smoothing applied to the outlines of the detected objects; a lower value will preserve more of the objects' morphological features |
There are two preset groups in the recipe: Colony Detection and Colony Refinement. Each group has three pre-configured parameter groupings to help you get started on the analysis. The default preset values are in the sections to follow.
The Colony Analysis recipe generates morphological and count measurements for detected colony regions. You can add additional measurements to the analysis results with the Measurement Tool and view measurement definitions on the Measurement Definitions page. The measurements generated by the recipe are as follows:
Morphology | Count |
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