Recipe parameters for Calcium Oscillation and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
---|---|---|---|---|
Detection | Contrast Threshold | 0 | 255 | Adjusts the detection sensitivity on the segmentation image;
A lower value will detect bigger and more objects |
Background Removal Factor (Remove Background only) | 0 | 100 | Adjusts the sensitivity of the background removal operation on the temporal projection image; a lower value will preserve larger objects and more background variations | |
Bright Spot Size (Remove Background only) | 0 | 50 | Adjusts the sensitivity of the bright peak enhancement operation; a lower value will enhance smaller, individual peaks | |
Tracking | Object Size | 0 | 1,000,000 (px2 or µm2) | Specifies the range of objects to be included in the analysis results based on the area of the detected objects |
Separation Factor | 0 | 100 | Adjusts the sensitivity of the object separation operation, a lower value will preserve larger objects with multiple intensity peaks |
There are two preset groups in the recipe: Detection and Tracking; each group has three pre-configured parameter groupings to help you get started on the analysis. The default preset values are given in the following subsections.
Before beginning the tutorial, please download the Calcium Oscillation Demo image. For information on how to select presets or modify parameter values, please refer to the tutorial on how to use the Recipe Console.
Load the demo image, “CaOscDemo.tif,” into Aivia.
Click Apply or Cancel on the Calibration Properties dialog to proceed with loading the image.
In the Recipe Console, click on the Recipe selection dropdown menu and select the Calcium Oscillation recipe.
Select the Low presets for the Detection and Tracking groups.
Click the Start button or press the F4
key on your keyboard to begin applying the recipe to the image.
The Calcium Oscillation recipe generates intensity and position measurements. You can add additional measurements to the analysis results by using the Measurement Tool in Aivia and find measurement definitions on the Measurement Definitions page. The measurements generated by the recipe are in the table below.
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