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The Recipe mode in the Batch Processor allows you to apply one (1) or more analysis recipes to multiple images simultaneously. The Batch Processor saves processed images along with Excel-readable measurement files for the analysis results to the designated output folder. Prior to applying an analysis recipe, you can load an image into Aivia and use the Recipe Console interface to adjust the recipe parameters for the best results.

Step-by-step guide

Before beginning the tutorial, please download the Nuclei Count Demo Images (NucleiCountDemo.zip). This tutorial uses the Nuclei Count recipe. Please refer to the documentation on the Nuclei Count recipe for more information about the recipe.

1. Unzip the demo file. There should be five (5) images total.

2. Load one (1) of the images into Aivia.

3. In the Recipe Console, click on the Recipe selection dropdown menu and select the Nuclei Count recipe.

4. Set the recipe parameters as shown below. You can view the results on the loaded image by clicking the Start button or pressing the F4 key on your keyboard to apply the recipe.

5. Go to the Analysis menu and select Batch & Cloud Processor; this launches the Batch & Cloud Processor window.

6. In the Create Job tab of the Batch & Cloud Processor, select Recipe mode.

7. In the dropdown menu next to the menu that has Recipe Apply selected, select the Nuclei Count recipe.

The alternative to Recipe Apply is Template Apply. In Template Apply mode, an object group on the current image is selected instead of a recipe from the adjacent dropdown menu. The recipe and recipe settings used to generate the selected object group as well as all measurements calculated for the object group are batch applied to the input images. See the Batch Processor page for more information on Template Apply as well as how to batch apply multiple recipes at once to a set of input images.

8. Click on the Add Local Folder button, navigate to the folder containing the unzipped demo images in the dialog that opens, and then click on Select Folder.

9. Click on the Browse button to the right of the Output Dir text field, navigate to the desired output location, and then click on Select Folder.

10. Assign the demo images as input by either dragging them from the Local Library section and dropping them into the Raw Input section or by selecting them in the Local Library section, right-clicking on the selected thumbnail(s), and choosing the Assign as Raw Input option.

11. Click Create to start the batch process. You can monitor progress in the Progress Queue tab of the Batch & Cloud Processor window and continue to use Aivia normally while the batch process is running.

12. When processing has completed, all processed images and exported measurements are in the designated output folder. The processed images have the “_Processed” suffix, and the exported measurements have the “_PrintToExcel” suffix.

13. You can open the processed images in Aivia to view the analysis results and open the data spreadsheets in Excel to view the data.

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