Parameters and Presets
Parameters
Recipe parameters for Colony Analysis and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
---|---|---|---|---|
Colony Detection | Colony Detection Sensitivity | 0 | 255 | Adjusts the sensitivity of the detection; a lower value will detect bigger and more objects; the MEF substrate and Matrigel substrate options let you select different detection routines depending on your plating substrate |
Colony Refinement | Minimum Colony Area | 0 | 1,000,000,000 | Specifies the minimum size for objects to be included in the analysis results based on the area of the detected objects |
Fill Holes Size | 0 | 1,000,000 | Adjusts the maximum size threshold for filling in gaps inside a detected object; a lower value will preserve more holes in the detection | |
Smoothing Factor | 0 | 100 | Adjusts the amount of smoothing applied to the outline of the detected objects; a lower value will preserve more of the object's morphological features |
Results
Colony Analysis results |