Parameters and Presets
Parameters
Recipe parameters for Nuclei Count and their descriptions are summarized in the table below.
Preset Group | Parameter Name | Min Value | Max Value | Description |
---|---|---|---|---|
Detection | Background Removal Factor (Remove Background only) | 0 | 100 | Adjusts the sensitivity of the background removal operation; a lower value will preserve larger objects and more background variations |
Contrast Threshold (Remove Background) Intensity Threshold (Skip Remove Background) | 0 | 255 (8-bit) 65,535 (16-bit) | Adjusts the detection sensitivity on the segmentation image
A lower value will detect bigger and more objects | |
Fill Holes Size | 0 | 1,000,000 | Adjusts the maximum size threshold for filling in gaps inside a detected object; a lower value will preserve more holes in the detection | |
Smoothing Factor | 0 | 100 | Adjusts the amount of smoothing applied to the outline of the detected objects; a lower value will preserve more of the object's morphological features | |
Nuclei Partition | Object Size | 0 | 1,000,000,000 | Specifies the range of objects to be included in the analysis results based on the area of the detected objects |
Separation Factor (Cell Partition only) | 0 | 100 | Adjusts the sensitivity of the object separation operation, a lower value will preserve larger objects with multiple intensity peaks |